Journal: Data in Brief

Article Title: Supporting data and methods for the characterization of iron oxide nanoparticles conjugated with pH-(low)-insertion peptide, testing their cytotoxicity and analyses of biodistribution in SCID mice bearing MDA-MB231 tumor ☆

doi: 10.1016/j.dib.2019.105062

Figure Lengend Snippet: Specifications Table

Article Snippet: How data were acquired , The morphology and size of nanoparticles were characterized by transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The hydrodynamic size of the nanoparticles was measured by dynamic light scattering (DLS). The immobilization of pHLIP on magnetic nanoparticles was confirmed by FTIR spectroscopy. The number of organic molecules bound to the nanoparticle surface was calculated based on Fourier-transform infrared (FTIR) spectroscopy, elemental analysis (CHN), inductively coupled plasma atomic emission spectroscopy (ICP-AES) and thermogravimetric analysis (TGA) data. Cell viability was measured by flow cytometry using membrane-impermeant DNA-binding fluorescent dyes. The concentration of iron in the tissue was measured by ICP-AES. The amount of eosinophils in tumor microsection was quantified by TEM. Perls' Prussian blue and hematoxylin & eosin stained tissue sections were imaged by light microscopy. Serum biochemical parameters were determined using clinical chemistry analyzer. Instruments: Nicolet 6700 Thermo FTIR-spectrometer (Thermo Scientific), CHN РЕ 2400 II automatic analyzer (Perkin Elmer), TGA/DSC1 instrument (Mettler Toledo), Zetasizer Nano ZS (Malvern), Merlin scanning electron microscope (SEM) (Carl Zeiss) equipped with Gemini column with Schottky field emission cathode as an electron source, ARCHITECT c4000 (Abbott Diagnostics, Lake Forest), BD Accuri-C6 flow cytometer (BD Bioscience), Horizontal tomographic scanner Biospec 117/16 USR (Bruker), iCAP 6300 Duo ICP Spectrometer (Thermo Scientific), Excelsior™ AS Tissue Processor (Thermo Scientific), HM 325 Rotary Microtome (Thermo Scientific), BOND-MAX Automated IHC/ISH Stainer (Leica Biosystems), Leica ultracut ultra-microtome (Leica), Transmission electron microscope JEM-1400 (JEOL). Data were analyzed using AxioVision, GraphPad Prism GraphPad Software..

Techniques: Transmission Assay, Electron Microscopy, Spectroscopy, Flow Cytometry, Concentration Assay, Staining, Light Microscopy, Microscopy, Software, Isolation, Gradient Centrifugation